Development of flexible new technologies for sugarcane bagasse and trash physical treatment, adaptable to downstream processes.

Study sugarcane raw bagasse depithing process and the applicability of its fractions in the enzymatic route for ethanol production.

Establishment of methodology for the characterization of the fractions obtained from sugarcane bagasse and trash.

Establishment of operational and project parameters to validate developed technologies and generate subsidies to scale up and to technical-economical viability studies.

Production of sugarcane bagasse and trash samples for studies at CTBE and at the Research groups.

Development of a technology for the deconstruction of the hemicellulose-cellulose-lignin matrix.

Development of processes to promote separation and dissolution of lignin, avoiding its chemical modification and dissolution of toxic phenol fraction.

Development of efficient processes for conversion of hemicellulose to pentoses, aiming at reduction of the formation of fermentation inhibitors compounds.

Establishment of mass and energy balances of the bagasse pretreatment processes for technical and economical evaluation.

Production of pretreated bagasse samples for studies at CTBE and at research groups.

Bioprocess development for enzymes production through submerged and semisolid fermentation using selected microorganisms.

Development and scaling up of biomass production bioprocesses of wild type or genetically modified microorganisms for hexoses and pentoses ethanol fermentation and for enzymes production.

Production of cellulolytic and ethanologenic microorganisms biomass to be used at CTBE and at the Research groups.

Development of the enzymatic hydrolysis process of pretreated sugarcane bagasse using hydrolases (exoglucanases, endoglucanases and β-glucosidase).

Development of the simultaneous enzymatic hydrolysis and ethanol fermentation process.

Production of bagasse hydrolysate for ethanol fermentation studies at CTBE and at the Research groups.

Process development of the treatment of final broth resulting from enzymatic hydrolysis or simultaneous enzymatic hydrolysis and fermentation.

Development of an appropriate route for detoxification of liquors from pretreatments and enzymatic hydrolysis.

Development of process recovering and purification of enzymatic complexes from submerged and semisolid fermentation, containing various enzymatic fractions (glucanases, xylanases and β-glucosidase).

Production of enzymatic complexes with different degrees of purity (together with PPDP3), to be used at CTBE and at Research groups.

Development of ethanol fermentation process for hydrolysis liquors containing hexoses combined (or not) with sugarcane juice and syrup.

Development of ethanol fermentation process using selected yeast or genetically modified microorganisms for fermentation of hexoses and pentoses (simultaneously or separated fermentation).

Establishment of operational parameters (mass and energy balances, stoichiometry and yield, kinetic and productivity) for technical and economical evaluation of the developed processes.